The role of Yes-associated protein (YAP) in Lytechinus pictus embryogenesis

David Aurand, Susquehanna University
Tiffany Beinlich, Susquehanna University
Cassandra Destefono, Susquehanna University
Nina Ngo, Susquehanna University
Margaret Peeler, Susquehanna University

Description

Regulation of cell division plays a critical role in animal development. The Hippo pathway helps regulate cell division through the process of phosphorylation of the Yes-associated protein (YAP). Although the major components of the pathway are present in sea urchins, little is known about the pathway's function in this species. Verteporfin, a known inhibitor of YAP, was used to determine the role of YAP in regulating cell proliferation and tissue development in Lytechinus pictus. Loss-of-function experiments were conducted using various concentrations of Verteporfin over a course of 48-hours after fertilization. This correlates to the level of YAP mRNA present during this stage of development. There were also specific effects on the number and distribution of skeletogenic mesenchyme cells. These results suggest that YAP plays a similar role in sea urchins, functioning to control cell division and tissue development, indicating evolutionary conservation of this pathway in animals.

 
Apr 25th, 5:00 PM Apr 25th, 6:00 PM

The role of Yes-associated protein (YAP) in Lytechinus pictus embryogenesis

Regulation of cell division plays a critical role in animal development. The Hippo pathway helps regulate cell division through the process of phosphorylation of the Yes-associated protein (YAP). Although the major components of the pathway are present in sea urchins, little is known about the pathway's function in this species. Verteporfin, a known inhibitor of YAP, was used to determine the role of YAP in regulating cell proliferation and tissue development in Lytechinus pictus. Loss-of-function experiments were conducted using various concentrations of Verteporfin over a course of 48-hours after fertilization. This correlates to the level of YAP mRNA present during this stage of development. There were also specific effects on the number and distribution of skeletogenic mesenchyme cells. These results suggest that YAP plays a similar role in sea urchins, functioning to control cell division and tissue development, indicating evolutionary conservation of this pathway in animals.