Event Title

The Role of Yes-Associated Protein (YAP) in L. pictus Embryogenesis

Faculty Advisor

Margaret Peeler

Start Date

25-4-2017 4:00 PM

End Date

25-4-2017 5:00 PM

Description

Regulation of cell division plays a critical role in animal development. The Hippo pathway helps regulate cell division through the process of phosphorylation of the Yes-associated protein (YAP). The Hippo pathway has been well characterized in several species, including flies and mammals. Although the major components of the pathway are present in sea urchins, little is known about the pathway's function in this species. Verteporfin, a known inhibitor of YAP, was used to determine the role of YAP in regulating cell proliferation and tissue development in Lytechinus pictus. Loss-of-function experiments were conducted using various concentrations of Verteporfin over a course of 48-hours after fertilization. YAP inhibition was shown to reduce cell number in a dosage dependent manner, with the strongest effect during and after gastrulation. This correlates to the level of YAP mRNA present during this stage of development. These results suggest that YAP plays a similar role in sea urchins, functioning to control cell division and tissue development, indicating evolutionary conservation of this pathway in animals.

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Apr 25th, 4:00 PM Apr 25th, 5:00 PM

The Role of Yes-Associated Protein (YAP) in L. pictus Embryogenesis

Regulation of cell division plays a critical role in animal development. The Hippo pathway helps regulate cell division through the process of phosphorylation of the Yes-associated protein (YAP). The Hippo pathway has been well characterized in several species, including flies and mammals. Although the major components of the pathway are present in sea urchins, little is known about the pathway's function in this species. Verteporfin, a known inhibitor of YAP, was used to determine the role of YAP in regulating cell proliferation and tissue development in Lytechinus pictus. Loss-of-function experiments were conducted using various concentrations of Verteporfin over a course of 48-hours after fertilization. YAP inhibition was shown to reduce cell number in a dosage dependent manner, with the strongest effect during and after gastrulation. This correlates to the level of YAP mRNA present during this stage of development. These results suggest that YAP plays a similar role in sea urchins, functioning to control cell division and tissue development, indicating evolutionary conservation of this pathway in animals.