Event Title

Insertion of Yellow-Fluorescent Protein(YFP) into the ELM1P Kinase

Presenter Information

Peter Lynch, Susquehanna University

Faculty Advisor

Courtney Thomas

Start Date

25-4-2017 12:00 PM

End Date

25-4-2017 1:00 PM

Description

In Saccharomyces cerevisiae, or budding yeast, proteins exist in the bud neck ring structure that are important for cellular morphology as well as cell division. In this neck ring structure the serine/threonine kinase Elm1p is located and is required for cell growth and division. In previous work Elm1p was co-purified with Gin4p, Nap1p, and Clb2p and the interaction of these proteins can be measured using fluorescence resonance energy transfer (FRET). Yellow fluorescent protein (YFP) plasmids were used as a template for polymerase chain reaction (PCR) to amplify the plasmids. These amplified plasmid cassettes were used to transform yeast cells. This transformation created Elm1p-YFP yeast cell strains. This strain will be used to verify the co-purification results with FRET.

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Apr 25th, 12:00 PM Apr 25th, 1:00 PM

Insertion of Yellow-Fluorescent Protein(YFP) into the ELM1P Kinase

In Saccharomyces cerevisiae, or budding yeast, proteins exist in the bud neck ring structure that are important for cellular morphology as well as cell division. In this neck ring structure the serine/threonine kinase Elm1p is located and is required for cell growth and division. In previous work Elm1p was co-purified with Gin4p, Nap1p, and Clb2p and the interaction of these proteins can be measured using fluorescence resonance energy transfer (FRET). Yellow fluorescent protein (YFP) plasmids were used as a template for polymerase chain reaction (PCR) to amplify the plasmids. These amplified plasmid cassettes were used to transform yeast cells. This transformation created Elm1p-YFP yeast cell strains. This strain will be used to verify the co-purification results with FRET.