Event Title

Comparison of Pointed RNAi Knockdown Phenotypes and the Quantification of Resulting mRNA Levels

Faculty Advisor

Pavithra Vivekanand

Start Date

25-4-2017 4:00 PM

End Date

25-4-2017 5:00 PM

Description

Pointed is an ETS family transcriptional activator that functions downstream of the EGFR RTK signaling pathway and is essential in the development and differentiation of several cell types. Previous research has shown that Pnt is needed for the differentiation of cell types within the eyes, somatic follicle cells, and heart cells of Drosophila melanogaster. We used three RNAi lines: Valium 20, Valium 10, and VDRC, to knockdown pnt mRNA expression and examined the phenotypic effects. Val20 is distinct since it generates a short hairpin RNA. Valium 10 and VDRC on the other hand produce long hairpin loop dsRNA. We found that Val20 produced the strongest phenotypes while Val10 and VDRC produced varying levels of severity. Based on these results the goal of our study is to determine whether the severity of phenotypes produced by the three RNAi lines correlates with the extent to which the pnt mRNA level is depleted.

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Apr 25th, 4:00 PM Apr 25th, 5:00 PM

Comparison of Pointed RNAi Knockdown Phenotypes and the Quantification of Resulting mRNA Levels

Pointed is an ETS family transcriptional activator that functions downstream of the EGFR RTK signaling pathway and is essential in the development and differentiation of several cell types. Previous research has shown that Pnt is needed for the differentiation of cell types within the eyes, somatic follicle cells, and heart cells of Drosophila melanogaster. We used three RNAi lines: Valium 20, Valium 10, and VDRC, to knockdown pnt mRNA expression and examined the phenotypic effects. Val20 is distinct since it generates a short hairpin RNA. Valium 10 and VDRC on the other hand produce long hairpin loop dsRNA. We found that Val20 produced the strongest phenotypes while Val10 and VDRC produced varying levels of severity. Based on these results the goal of our study is to determine whether the severity of phenotypes produced by the three RNAi lines correlates with the extent to which the pnt mRNA level is depleted.