Event Title

Adipogenesis and the Effect of Saccharin on Transcription Factor PPARy

Presenter Information

James Kelly

Faculty Advisor

Dr. Tom Peeler

Start Date

24-4-2018 12:00 PM

End Date

24-4-2018 1:00 PM

Description

Over 65% of the American population is overweight or obese. This weight gain can be attributed to over eating, sedentary lifestyles, and other factors, which result in the formation of excess adipose tissue. Adipocyte (fat cell) formation from stem cells is stimulated by transcription factors, such as PPARγ and C/EBPa, that initiate the process of adipogenesis (fat cell development). The artificial sweetener saccharin has been shown to enhance the process of adipogenesis in the 3T3-L1 cell model system of fat cell development. Our research is investigating the relationship between the artificial sweetener saccharin and the transcription factor PPARγ. We hypothesized that saccharin directly activates PPARγ, thereby stimulating adipogenesis. We have transfected DNA plasmids containing a luciferase reporter gene attached to a PPARγ promoter response element into 3T3-L1 cells containing PPARγ, and tested whether saccharin enhanced transcription of the reporter gene. The results of this research will improve our understanding of the role of saccharin in enhancing fat cell development in the 3T3-L1 model system.

This document is currently not available here.

Share

COinS
 
Apr 24th, 12:00 PM Apr 24th, 1:00 PM

Adipogenesis and the Effect of Saccharin on Transcription Factor PPARy

Over 65% of the American population is overweight or obese. This weight gain can be attributed to over eating, sedentary lifestyles, and other factors, which result in the formation of excess adipose tissue. Adipocyte (fat cell) formation from stem cells is stimulated by transcription factors, such as PPARγ and C/EBPa, that initiate the process of adipogenesis (fat cell development). The artificial sweetener saccharin has been shown to enhance the process of adipogenesis in the 3T3-L1 cell model system of fat cell development. Our research is investigating the relationship between the artificial sweetener saccharin and the transcription factor PPARγ. We hypothesized that saccharin directly activates PPARγ, thereby stimulating adipogenesis. We have transfected DNA plasmids containing a luciferase reporter gene attached to a PPARγ promoter response element into 3T3-L1 cells containing PPARγ, and tested whether saccharin enhanced transcription of the reporter gene. The results of this research will improve our understanding of the role of saccharin in enhancing fat cell development in the 3T3-L1 model system.