Event Title

Chemical composition of an acetone extract of Vitis riparia

Presenter Information

Gustin Reynolds

Faculty Advisor

Dr. Geneive Henry

Start Date

24-4-2018 12:00 PM

End Date

24-4-2018 1:00 PM

Description

To determine if the riverbank grape, vitis riparia, contained useful polyphenols or resveratrol oligomer units, a large sample of ground leaves and stems were extracted and fractioned with dry celite packing and the use of acetone in solid phase chromatography. Fractions were isolated based on polarity and Thin Layer Chromatography (TLC) of resulting fractions allowed further separation. Combiflash solid phase chromatography was used to further separate like fractions, which were dried and identified using NMR spectroscopy. Data of separated compounds revealed the presence of several long fatty acid chains, waxes, phetophytin A, and sitosterol, the latter being a member of phytosterol compounds that has potential to decrease blood cholesterol levels and aid in the treatment of benign prostatic hyperplasia. Unique fractions have since been found and are being identified. Attempts to separate polyphenolic compounds from vitis riparia have not yet been fruitful, but all fractions have yet to be identified.

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Apr 24th, 12:00 PM Apr 24th, 1:00 PM

Chemical composition of an acetone extract of Vitis riparia

To determine if the riverbank grape, vitis riparia, contained useful polyphenols or resveratrol oligomer units, a large sample of ground leaves and stems were extracted and fractioned with dry celite packing and the use of acetone in solid phase chromatography. Fractions were isolated based on polarity and Thin Layer Chromatography (TLC) of resulting fractions allowed further separation. Combiflash solid phase chromatography was used to further separate like fractions, which were dried and identified using NMR spectroscopy. Data of separated compounds revealed the presence of several long fatty acid chains, waxes, phetophytin A, and sitosterol, the latter being a member of phytosterol compounds that has potential to decrease blood cholesterol levels and aid in the treatment of benign prostatic hyperplasia. Unique fractions have since been found and are being identified. Attempts to separate polyphenolic compounds from vitis riparia have not yet been fruitful, but all fractions have yet to be identified.