Event Title

Investigating the Effectiveness of long hairpin and short hairpin EGFR RNAi in the Drosophila Melanogaster

Faculty Advisor

Dr. Pavithra Vivekanand

Start Date

23-4-2019 5:20 PM

End Date

23-4-2019 5:40 PM

Description

Normal development of Drosophila wing, eye, and midline glia depend on proper function of Epidermal Growth Factor Receptor (EGFR). EGFR is a highly conserved receptor tyrosine kinase that functions widely in drosophila and mammals. We are systematically investigating the effectiveness of four RNAi lines in how well they knock down the expression of egfr in the study tissues. Our results show that we do not consistently see short hairpin RNAi being more effective than long hairpin RNAi, as we hypothesized. In the eyes and wings, VDRC (long hairpin) was the most severe, and valium 10 (long hairpin) was the least severe, with the two short hairpin RNAi lines being in the middle. The midline glia knockdowns are ongoing, and conclusive results are in progress. Based on current observations, we cannot say that short hairpin RNAi is more effective than long hairpin RNAi, in the tissue environments studied.

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Apr 23rd, 5:20 PM Apr 23rd, 5:40 PM

Investigating the Effectiveness of long hairpin and short hairpin EGFR RNAi in the Drosophila Melanogaster

Normal development of Drosophila wing, eye, and midline glia depend on proper function of Epidermal Growth Factor Receptor (EGFR). EGFR is a highly conserved receptor tyrosine kinase that functions widely in drosophila and mammals. We are systematically investigating the effectiveness of four RNAi lines in how well they knock down the expression of egfr in the study tissues. Our results show that we do not consistently see short hairpin RNAi being more effective than long hairpin RNAi, as we hypothesized. In the eyes and wings, VDRC (long hairpin) was the most severe, and valium 10 (long hairpin) was the least severe, with the two short hairpin RNAi lines being in the middle. The midline glia knockdowns are ongoing, and conclusive results are in progress. Based on current observations, we cannot say that short hairpin RNAi is more effective than long hairpin RNAi, in the tissue environments studied.