Event Title

Growth rates of M. centralius, M. silvanus, and M. ruber in methanol and carbon-depleted media

Presenter Information

Sean Murray, Susquehanna University

Faculty Advisor

Dr. Wade Johnson

Start Date

23-4-2019 12:00 PM

End Date

23-4-2019 1:00 PM

Description

Metathermus centralius, unlike the closely related Metathermus silvanus and Meiothermus ruber, contains a genetic pathway that potentially grants M. centralius the ability to digest methanol on minimal media. M. centralius, M. silvanus, and M. ruber were cultivated in media lacking several carbon sources and monitored for growth. Depriving them of nutrients revealed strong growth of M. centralius in standard media, with positive growth in single, double, and triple carbon-lacking media. M. silvanus and M. ruber seemed to lack the ability to grow in minimal media. Addition of 600 mM and 900 mM methanol to standard media reduces growth rates of all three but, under current conditions, does not yet select for M. centralius over the control strains. Future analyses of the bacteria in carbon-depleted media with supplemental methanol are predicted to reveal stronger M. centralius growth when compared to M. silvanus, and M. ruber.

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Apr 23rd, 12:00 PM Apr 23rd, 1:00 PM

Growth rates of M. centralius, M. silvanus, and M. ruber in methanol and carbon-depleted media

Metathermus centralius, unlike the closely related Metathermus silvanus and Meiothermus ruber, contains a genetic pathway that potentially grants M. centralius the ability to digest methanol on minimal media. M. centralius, M. silvanus, and M. ruber were cultivated in media lacking several carbon sources and monitored for growth. Depriving them of nutrients revealed strong growth of M. centralius in standard media, with positive growth in single, double, and triple carbon-lacking media. M. silvanus and M. ruber seemed to lack the ability to grow in minimal media. Addition of 600 mM and 900 mM methanol to standard media reduces growth rates of all three but, under current conditions, does not yet select for M. centralius over the control strains. Future analyses of the bacteria in carbon-depleted media with supplemental methanol are predicted to reveal stronger M. centralius growth when compared to M. silvanus, and M. ruber.