Event Title

Effect of Raspberry Ketone on Adipogenesis and Lipolysis in 3T3-L1 Mouse Preadipocytes

Faculty Advisor

Dr. Thomas Peeler

Start Date

23-4-2019 4:00 PM

End Date

23-4-2019 5:00 PM

Description

The prevalence of obesity in the United States has seen a sharp increase since the mid-twentieth century, which has led to increased research on the process of adipogenesis, or the differentiation of preadipocytes to mature adipocytes. The 3T3-L1 cell line is a mouse preadipocyte used to study the underlying mechanisms of adipogenesis. Raspberry ketone (4-(4-hydroxyphenyl) butan-2-one; RK) is a naturally occurring compound found in red raspberries and is often present in weight loss supplements. RK has a structure that is similar to other compounds, such as capsaicin and synephrine, that are known to both inhibit adipogenesis and enhance lipolysis. To test the effect of RK on adipogenesis, 3T3-l1 preadipocytes were treated with an adipogenesis inducing treatment containing IBMX, insulin, and dexamethasone. RK solutions of 200 mM, 300 mM, and 400 mM RK were added to the adipogenesis inducing treatment, and the cells were allowed to mature for eight days. In order to test rates of lipolysis, the cells were treated with the adipogenesis inducing treatment for eight days, and then given the same 200 mM, 300 mM, and 400 mM RK treatment on day eight. The cells were given this treatment for six days. To quantify rates of adipogenesis and lipolysis, Oil Red O staining was used. Western blotting was used to identify the presence of two key proteins involved in adipogenesis and lipolysis, such as Fatty Acid Binding Protein 4 (FABP4) and Peroxisome Proliferator-activated Receptor Gamma (PPARγ). RK is expected to both inhibit adipogenesis and increase lipolysis in a concentration dependent manner, resulting in lower levels of FABP4 and PPARγ expression. Gaining a deeper understanding of the effects of different compounds such as RK on obesity may allow the translation of these lab practices to a clinical setting and help combat the growing obesity epidemic.

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Apr 23rd, 4:00 PM Apr 23rd, 5:00 PM

Effect of Raspberry Ketone on Adipogenesis and Lipolysis in 3T3-L1 Mouse Preadipocytes

The prevalence of obesity in the United States has seen a sharp increase since the mid-twentieth century, which has led to increased research on the process of adipogenesis, or the differentiation of preadipocytes to mature adipocytes. The 3T3-L1 cell line is a mouse preadipocyte used to study the underlying mechanisms of adipogenesis. Raspberry ketone (4-(4-hydroxyphenyl) butan-2-one; RK) is a naturally occurring compound found in red raspberries and is often present in weight loss supplements. RK has a structure that is similar to other compounds, such as capsaicin and synephrine, that are known to both inhibit adipogenesis and enhance lipolysis. To test the effect of RK on adipogenesis, 3T3-l1 preadipocytes were treated with an adipogenesis inducing treatment containing IBMX, insulin, and dexamethasone. RK solutions of 200 mM, 300 mM, and 400 mM RK were added to the adipogenesis inducing treatment, and the cells were allowed to mature for eight days. In order to test rates of lipolysis, the cells were treated with the adipogenesis inducing treatment for eight days, and then given the same 200 mM, 300 mM, and 400 mM RK treatment on day eight. The cells were given this treatment for six days. To quantify rates of adipogenesis and lipolysis, Oil Red O staining was used. Western blotting was used to identify the presence of two key proteins involved in adipogenesis and lipolysis, such as Fatty Acid Binding Protein 4 (FABP4) and Peroxisome Proliferator-activated Receptor Gamma (PPARγ). RK is expected to both inhibit adipogenesis and increase lipolysis in a concentration dependent manner, resulting in lower levels of FABP4 and PPARγ expression. Gaining a deeper understanding of the effects of different compounds such as RK on obesity may allow the translation of these lab practices to a clinical setting and help combat the growing obesity epidemic.