Event Title

Water-soluble porphyrins that show specificity towards G-quadruplex DNA: microwave synthesis, characterization, and spectroscopic study

Presenter Information

Kara Pytko, Susquehanna University

Faculty Advisor

Dr. Swarna Basu

Start Date

23-4-2019 12:00 PM

End Date

23-4-2019 1:00 PM

Description

Porphyrins are heterocyclic organic molecules that can be used as highly specific fluorescent DNA probes. A series of water-soluble, non-aggregating porphyrins have been synthesized and characterized using NMR spectroscopy and silica gel thin layer chromatography. Previous synthesis methods have been improved using microwave irradiation to directly heat samples at the molecular level, allowing for faster reaction times. The porphyrins will be used to detect G-quadruplexes, a guanine-rich form of DNA found in the telomeric region of the chromosome. G-quadruplexes inhibit the enzyme telomerase, which causes cancer cell proliferation, making them targets for cancer research. Interactions between porphyrins and G-quadruplexes will be characterized using surface-enhanced Raman scattering (SERS), UV-Vis, and fluorescence spectroscopy. Additionally, interactions between metal complexes and quadruplex DNA were characterized using SERS and fluorescence spectroscopy. Results from both techniques show that the zinc complex preferentially binds to 17mer DNA.

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Apr 23rd, 12:00 PM Apr 23rd, 1:00 PM

Water-soluble porphyrins that show specificity towards G-quadruplex DNA: microwave synthesis, characterization, and spectroscopic study

Porphyrins are heterocyclic organic molecules that can be used as highly specific fluorescent DNA probes. A series of water-soluble, non-aggregating porphyrins have been synthesized and characterized using NMR spectroscopy and silica gel thin layer chromatography. Previous synthesis methods have been improved using microwave irradiation to directly heat samples at the molecular level, allowing for faster reaction times. The porphyrins will be used to detect G-quadruplexes, a guanine-rich form of DNA found in the telomeric region of the chromosome. G-quadruplexes inhibit the enzyme telomerase, which causes cancer cell proliferation, making them targets for cancer research. Interactions between porphyrins and G-quadruplexes will be characterized using surface-enhanced Raman scattering (SERS), UV-Vis, and fluorescence spectroscopy. Additionally, interactions between metal complexes and quadruplex DNA were characterized using SERS and fluorescence spectroscopy. Results from both techniques show that the zinc complex preferentially binds to 17mer DNA.