Event Title

Effects of 1,3-Dimethylamylamine (DMAA) on 3T3-L1 Cell Adipocytes

Faculty Advisor

Dr. Tom Peeler

Start Date

23-4-2019 5:00 PM

End Date

23-4-2019 6:00 PM

Description

Obesity rates have tripled in the United States since the 1970’s and currently, about 40% of adults living in the U.S are obese. Current methods to combat obesity include dieting and exercise. A stimulant, 1,3-dimethylamylamine (DMAA), was previously used as a dietary supplement to increase weight loss and athletic performance. This experiment was performed to explore the effects of 1,3-dimethylamylamine on lipid metabolism in mature adipocytes developed from 3T3-L1 cells. 3T3-L1 cells are mouse preadipocytes that can be converted to mature adipocytes by treatment with 3-isobutyl-1-methylxanthine (IBMX), dexamethasone, and insulin. Mature adipocytes were treated with 0.01 mM and 0.10 mM DMAA for 6 days, and lipid levels were quantified using Oil Red O staining. Preliminary findings suggest that 0.01 mM and 0.10 mM DMAA have little effect on lipolysis of lipids in the mature adipocytes compared to controls. We plan to test higher concentrations of DMAA (0.05 and 0.2 mM) and observe the effects on lipolysis. Learning more about the effects of DMAA on lipolysis at the cellular level may lead to a better understanding of fat tissue development and obesity.

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Apr 23rd, 5:00 PM Apr 23rd, 6:00 PM

Effects of 1,3-Dimethylamylamine (DMAA) on 3T3-L1 Cell Adipocytes

Obesity rates have tripled in the United States since the 1970’s and currently, about 40% of adults living in the U.S are obese. Current methods to combat obesity include dieting and exercise. A stimulant, 1,3-dimethylamylamine (DMAA), was previously used as a dietary supplement to increase weight loss and athletic performance. This experiment was performed to explore the effects of 1,3-dimethylamylamine on lipid metabolism in mature adipocytes developed from 3T3-L1 cells. 3T3-L1 cells are mouse preadipocytes that can be converted to mature adipocytes by treatment with 3-isobutyl-1-methylxanthine (IBMX), dexamethasone, and insulin. Mature adipocytes were treated with 0.01 mM and 0.10 mM DMAA for 6 days, and lipid levels were quantified using Oil Red O staining. Preliminary findings suggest that 0.01 mM and 0.10 mM DMAA have little effect on lipolysis of lipids in the mature adipocytes compared to controls. We plan to test higher concentrations of DMAA (0.05 and 0.2 mM) and observe the effects on lipolysis. Learning more about the effects of DMAA on lipolysis at the cellular level may lead to a better understanding of fat tissue development and obesity.