Event Title

Investigating the Effect of EGFR RNAi on Ventral Midline Glial Cells in Drosophila Melanogaster

Faculty Advisor

Dr. Pavithra Vivekanand

Start Date

23-4-2019 12:00 PM

End Date

23-4-2019 1:00 PM

Description

In Drosophila melanogaster, the EGFR pathway is known to play a role in the organization of the central nervous system. More specifically, the ventral midline glial development during embryogenesis and the organization of the axonal scaffold, a ladder like structure comprised of longitudinal connectives and segmental commissures. The EGFR pathway activates the Ras/MAPK kinase cascade that phosphorylates the transcriptional activator, PntP2 leading to the differentiation of midline glia. In wild type embryos, the midline glia are required for ensheathing and therefore the separation of commissures. We expressed EGFR RNAi in the ventral midline glial cells using four RNAi lines: two Valium20 lines which produce short-hairpin RNAs, as well as Valium10 and VDRC which produce both long-hairpin RNAs. Using these four lines we will assess the effectiveness of each RNAi lines to affect the differentiation of the midline glia by and determine if there is a greater effect on the development of midline glial cells using either short or the long hairpin RNAi. The single-minded-gal4 line will be used to knockdown the expression of egfr mRNA in midline glia, which allows us to specifically target this area without perturbing development of other tissues in the embryos of Drosophila melanogaster.

This document is currently not available here.

Share

COinS
 
Apr 23rd, 12:00 PM Apr 23rd, 1:00 PM

Investigating the Effect of EGFR RNAi on Ventral Midline Glial Cells in Drosophila Melanogaster

In Drosophila melanogaster, the EGFR pathway is known to play a role in the organization of the central nervous system. More specifically, the ventral midline glial development during embryogenesis and the organization of the axonal scaffold, a ladder like structure comprised of longitudinal connectives and segmental commissures. The EGFR pathway activates the Ras/MAPK kinase cascade that phosphorylates the transcriptional activator, PntP2 leading to the differentiation of midline glia. In wild type embryos, the midline glia are required for ensheathing and therefore the separation of commissures. We expressed EGFR RNAi in the ventral midline glial cells using four RNAi lines: two Valium20 lines which produce short-hairpin RNAs, as well as Valium10 and VDRC which produce both long-hairpin RNAs. Using these four lines we will assess the effectiveness of each RNAi lines to affect the differentiation of the midline glia by and determine if there is a greater effect on the development of midline glial cells using either short or the long hairpin RNAi. The single-minded-gal4 line will be used to knockdown the expression of egfr mRNA in midline glia, which allows us to specifically target this area without perturbing development of other tissues in the embryos of Drosophila melanogaster.