Event Title

Underground Mine Fire: Increased Temperatures Altering Abundance of Sulfur Cycle Related Genes

Presenter Information

Madison ReedFollow

Faculty Advisor

Dr. Tammy Tobin

Start Date

28-4-2020 12:00 AM

End Date

28-4-2020 12:00 AM

Description

Centralia, Pennsylvania is a town that was affected by an underground coal mine fire in the 60s. It has been burning ever since and is expected to continue burning for another 100 years. As this coal fire burns, it heats, and deposits oxidized sulfur products into the overlying soils and releases sulfur dioxide into the air. This, in turn, causes environmental dangers such as amplified acid rain and acid mine runoff into neighboring water sources. Soil samples have been collected from affected and unaffected sites in Centralia since the fall of 2014. Metagenomic analyses are being performed on DNA extracted from these samples in order to correlate the relative abundance of genes involved in sulfur-cycling to soil sulfur concentrations. Real-time PCR conditions are also being developed to allow us to assay the expression of these genes. Ultimately, this work will help us determine how sulfur-cycling bacteria are influencing the environmental impacts of the coalmine fire and may lead to new strategies for bioremediation.

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Apr 28th, 12:00 AM Apr 28th, 12:00 AM

Underground Mine Fire: Increased Temperatures Altering Abundance of Sulfur Cycle Related Genes

Centralia, Pennsylvania is a town that was affected by an underground coal mine fire in the 60s. It has been burning ever since and is expected to continue burning for another 100 years. As this coal fire burns, it heats, and deposits oxidized sulfur products into the overlying soils and releases sulfur dioxide into the air. This, in turn, causes environmental dangers such as amplified acid rain and acid mine runoff into neighboring water sources. Soil samples have been collected from affected and unaffected sites in Centralia since the fall of 2014. Metagenomic analyses are being performed on DNA extracted from these samples in order to correlate the relative abundance of genes involved in sulfur-cycling to soil sulfur concentrations. Real-time PCR conditions are also being developed to allow us to assay the expression of these genes. Ultimately, this work will help us determine how sulfur-cycling bacteria are influencing the environmental impacts of the coalmine fire and may lead to new strategies for bioremediation.