Presenter Information

Jaden CarperFollow

Faculty Advisor

Dr. Thomas Peeler

Start Date

27-4-2021 12:00 AM

End Date

27-4-2021 12:00 AM

Description

The prevalence of obesity in the United States has increased from 30.5% in 1999 to 42.4% in 2018. Fat cells are created through a process called adipogenesis; in adipogenesis, a preadipocyte, which is an undifferentiated fibroblast, is differentiated into an adipocyte, which is a mature fat cell. Peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα) are two transcription factors that play crucial roles in the process of adipogenesis. Prior research has shown that coffee extract can inhibit the adipogenesis pathway and decrease the number of adipocytes formed in mice. Previous studies have also published conflicting results concerning whether caffeine is the compound in the coffee responsible for the inhibition of adipogenesis. 3T3-L1 preadipocytes are embryonic fibroblasts derived from mice and are used as a model system to study adipogenesis in vitro. 3T3-L1 preadipocytes were differentiated into adipocytes using MDI induction media (IBMX, dexamethasone, and insulin), insulin media, and 10% FBS/DMEM. To determine the role of coffee on adipogenesis, 5% Starbucks Pike Place Roast caffeinated, or decaffeinated coffee extract were added during each differentiation step. Oil Red O staining was used to quantify the levels of adipogenesis in each of the treatments. Treatments with the coffee extracts inhibited the rate of adipogenesis compared to cells treated with the normal differentiation, indicating while caffeine had no effect, some compounds in the coffee extract inhibited adipogenesis in 3T3-L1 cells. By learning more about the process of adipogenesis, we may better understand causes of and develop treatments for obesity.

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Apr 27th, 12:00 AM Apr 27th, 12:00 AM

The Effects of Coffee Extract on Adipogenesis

The prevalence of obesity in the United States has increased from 30.5% in 1999 to 42.4% in 2018. Fat cells are created through a process called adipogenesis; in adipogenesis, a preadipocyte, which is an undifferentiated fibroblast, is differentiated into an adipocyte, which is a mature fat cell. Peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα) are two transcription factors that play crucial roles in the process of adipogenesis. Prior research has shown that coffee extract can inhibit the adipogenesis pathway and decrease the number of adipocytes formed in mice. Previous studies have also published conflicting results concerning whether caffeine is the compound in the coffee responsible for the inhibition of adipogenesis. 3T3-L1 preadipocytes are embryonic fibroblasts derived from mice and are used as a model system to study adipogenesis in vitro. 3T3-L1 preadipocytes were differentiated into adipocytes using MDI induction media (IBMX, dexamethasone, and insulin), insulin media, and 10% FBS/DMEM. To determine the role of coffee on adipogenesis, 5% Starbucks Pike Place Roast caffeinated, or decaffeinated coffee extract were added during each differentiation step. Oil Red O staining was used to quantify the levels of adipogenesis in each of the treatments. Treatments with the coffee extracts inhibited the rate of adipogenesis compared to cells treated with the normal differentiation, indicating while caffeine had no effect, some compounds in the coffee extract inhibited adipogenesis in 3T3-L1 cells. By learning more about the process of adipogenesis, we may better understand causes of and develop treatments for obesity.

 

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